A macromolecule serving as a catalyst...

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I have assignment that a tutor did and it needs to be polished up. The catch is it is due in 2 hours and I don't have time to make it sound more like a lab report, for example I hypothesis bla bl abla Anybody interested? The experiment is attached along with direction and answers
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Biology 124L Fall 2014
Lab Report 2: Enzymes: Answer Sheet
Please type your answers into this answer sheet, and submit only the completed answer sheet to your TA.
Due Date: The beginning of your scheduled lab section during the week of September 15 th.
Name: _________________________________________ Section
1a
1b
A macromolecule serving as a catalyst, a chemical agent that increases the rate and
decreasing energy activation of a reaction without being consumed by the reaction. Most
enzymes are proteins.
Organism produce thousand enzyme that act as catalyst of many reaction that constitute
organism metabolism. Those many enzymes are so important keeping biochemistry of
organism that sustains life of organism can happen at average temperature. Without enzyme
many reactions will happen slowly and cannot sustain life. That why organism produce lot
of enzyme
Tyrosinase is an enzyme that catalyst reaction that convert pryocatecol into hydroxyquinone
(yellow-brown liquid) and responsible for brown or bruises in fruit and vegetables.
Hydoxyquinone is a natural antibiotic that prevent infection of injured plant.
2
-To test substrate-specific of tyrosine
-To know what color that indicate reaction mediated by tyrosine happen or not happen
3
This experiment is important to obtain indicator that indicate activity of enzyme so when we
make future experiment, we can check activity of enzyme in modified variable condition
like different pH, different temperature and adding inhibitor
4a
The optimal temperature of tyrosine activity are between 30-34 (little bit lower from body
temperature
-In test tube I (refrigerator temperature) initial color are clear and final color are clear. Those
colors indicate no hydroxyquinone are produced
- In test tube II (room temperature) initial color are light yellow and final color are yellow.
Those colors indicate average amount hydroxyquinone are produced
4b
- In test tube III (35 celcius) initial color are yellow and final color are dark yellow. Those
colors indicate high amount hydroxyquinone are produced.
- In test tube IV (60 celcius) initial color are clear and final color areclear. Those colors
indicate no amount hydroxyquinone are produced.
4c
According to result, experiment result support hypothesis that states optimal temperature of
tyrosine activity are between 30-34 (little bit lower from body temperature). In test tube III
(35 celcius) initial color are yellow and final color are dark yellow. Those colors indicate
high amount hydroxyquinone are produced thus indicate high activity of enzyme.
5a
The higher concentration of enzyme, the higher activity enzyme converting pryocatecol into
hydoxyquinone.
-In test tube I (Lowest concentration of enzyme), initial color is light yellow and color after
5 minutes is yellow. Those colors indicate small amount hydroxyquinone are produced.
5b
--In test tube II (Medium concentration of enzyme), initial color is light yellow and color
after 5 minutes is dark yellow. Those colors indicate medium amount hydroxyquinone are
produced.
-In test tube III (Highest concentration of enzyme), initial color is dark yellow and color
after 5 minutes is dark yellow to pink. Those colors indicate high amount hydroxyquinone
are produced.
5c
According to result, experiment result support hypothesis that state the higher concentration
of enzyme, the higher activity enzyme converting pryocatecol into hydoxyquinone. In test
tube I (Lowest concentration of enzyme) only small amount hydroxyquinone are produced
thus indicate low activity of enzyme. In test tube III (Highest concentration of enzyme)
high amount hydroxyquinone are produced thus indicate high activity of enzyme
6a
The optimum pH of Tyrosine enzyme is at neutral pH (pH 7)
-In test tube I (pH 4) initial color are clear and final color are light yellow. Those colors
indicate small amount hydroxyquinone are produced
6b
- In test tube II (pH 7) initial color are light yellow and final color are yellow. Those colors
indicate average amount hydroxyquinone are produced
- In test tube III (pH 10) initial color are yellow and final color are light yellow. Those
colors indicate small amount hydroxyquinone are produced.
6c
7a
According to result, experiment result support hypothesis that states optimal pH of Tyrosine
enzyme is at neutral pH (pH 7). In test tube II (pH 7) initial color are light yellow and final
color are yellow. Those colors indicate average amount hydroxyquinone are produced thus
indicate average activity of enzyme. pH 7 is best pH if compared to acidic pH (test tube I)
and basic pH (test tube III)
Adding inhibitors will decrease tyrosine enzyme activity converting pryocatecol into
hydroxyquinone
- In test tube I (without phenylthiourea) initial color are yellow and final color are dark
yellow. Those colors indicate high amount hydroxyquinone are produced.
7b
-In test tube I (with phenylthiourea) initial color are clear and final color are clear. Those
colors indicate no hydroxyquinone are produced
7c
According to result, experiment result support hypothesis that states adding inhibitors will
decrease tyrosine enzyme activity converting pryocatecol into hydroxyquinone. If we
compare experiment result of test tube I and test tube II, test tube I (without phenylthiourea)
show high activity of enzyme (indicated by dark yellow color) than test tube II (with
phenylthiourea) that show low activity of enzyme (indicated by clear color)
8a
Optimal temperature of catalase enzyme are between 33-36 (human body temperature)
8b
Higher because adaptation of thermophilic bacteria shift its catalase optimum temperature to
higher temperature so enzyme still can work in hot environment where themophilic bacteria
live
Temperature can affect activity of tyrosine enzyme by affecting chemical bonding (e.g
hydrogen bond, disuphidebridge) between amino acids that constitute tyrosine. Chemical
bonding between amino acids of protein determine final shape and active site of enzyme.
8c
Increasing temperature will break chemical bonding of amino acids thus denature the
enzyme. The denatured enzyme cannot catalyst reaction thus decrease activity of enzyme
converting substrate to product
9a
pH optimum of tyrosine enzyme is at neutral ph (pH 7) and it supported by result from
experiment 4.
9b
pH optimum of tyrosine enzyme from potato that were grown in Idaho island is at basic
condition (pH 8). This change are caused by adaptation of potato so potato can still live and
tyrosine enzyme still work in basic condition
9c
pH optimum is at acidic. pH optimum are differ depending growing condition because
regulation optimum ph are needed so plant can fit in place where they live and keep their
existence in earth
10a
Adding competitive inhibitor will decrease activity of enzyme converting its substrate to
product by binding to active site of enzyme so substrate cannot bind to enzyme thus reaction
is blocked. Competitive inhibitor also decrease Km of enzyme thus decrease efficiency o
enzyme
10b
Competitive inhibitors do not completely block the production of the product. The process
blocking active site of enzyme by inhibitors are reversible so by the time enzyme can bind
again to substrate and convert it to product
10c
The color of test tube will become yellow

 

Solution ID:351010 | This paper was updated on 26-Nov-2015

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