Typical cell size for bacteria...

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Summer2014CellBio202
Thesearestudyquestionsthatcoverthefirstweekofclass.Theycoverallthematerialemphasized,andthe
contentofthefirstportionofexam1.Ifyoucananswerthesewithouthelp,youshoulddowellonthat
partoftheexam.
Whatisatypicalcellsizeforbacteria?Foreukaryotes?
Whatcellstructuresarecharacteristicofeukaryotesvs.prokaryotes?Animalsvs.plants?Whatfeaturesare
presentinallcells?
Whatiscelltheory?
Howcanweseecells?Individualcells?Cellsinsolidtissues?
Whatarethedifferenttypesoflightmicroscopy,howdopreparationsforeachdiffer,andwhatarethe
advantagesorusesofeach?
WhatlimitsthefeaturesobservableinLM?Howcanweidentifysmallerobject?
HowaresolidtissuesamplespreppedforLM?ForTEM?ForSEM?WhatarethedifferencesbetweenLMand
TEMtissuepreparations?(steps,dimensions,staining?)
HowdoesconfocalLMdifferfromstandard(includingfluorescence)LM,intermsofresults?
WhydoesEMhavehigherresolutionthanLM?
HowisSEMdifferentfromTEM?Whataretheadvantagesofeach?
Howcanyouobtain3DdescriptionsofcellswithLM?WithEM?
Whatkindsofmagnifications(LMorEM)areneededtoseeordescribe/measurevariouscellfeatures?
Whatarearchaea?
Whatarethecharacteristicfeaturesandgeneralpurposesofnuclie?RoughER?SmoothER?Golgi?
Lysosomes?Vesicles?Cytoskeleton?Mitochondria?
Whatareexocytosisandendocytosis?
Whatarethemajorcomponentsofcell?
Howdomostmacromoleculesinteractwitheachother?
Whatarenoncovalentinteractions?Howaretheyaffectedbytheirenvironment?
Howaremacromoleculesmade?Whatkindofreaction?Howaretheybrokendown?
Whatarethereactionsthatmakemacromoleculesandotherneededmoleculesincells?
Whatarethedualpurposesofcatabolism?
Whyarepolysaccharidesmadeincells?
Whatareorganicmolecules,andwhattheirsource?
Howisenergyobtainedfromorganicmolecules?Whataretheformsofthatenergy?Howiseachtypestored?
Howiseachtypeofenergyusedinthecell?
Whatareactivatedcarriers?Whatchangesinthem(inADPandNAD+)tomakethemactivated?
WhatkindsofbondscouplephosphatesinATP?Whatarethephosphatesusedtodoincells?
WhatarethestructuralandfunctionaldifferencesbetweenNADHandNADPH?
WhatisthefunctionofCoenzymeAincells?
Whatisthegeneralstructureofthebuildingblocksofproteins?Howaretheycombined,andwhatkindofbond
dotheyform?
Whataretheclassesofaminoacids?BeabletoidentifytheclassofanyAAfromitsstructure.
WhatarethefunctionaldifferencesoftheAAsintermsoftheinteractionsinwhichtheycanparticipate?
WhatarethetypesofHbondsthatcanoccurinapolypeptide?
Whichonesareusedtomakesecondarystructures?
Whatarethedifferencesbetweenalphaandbetastructures?Thesimilarities?
Whatmakesapolypeptideaprotein?
Whatistheoverallarrangementofmostproteins?Whatinteractionsarepresentindifferentparts?Howdoes
thatinfluencethestrengthoftheinteracitons?
Howisamembranespanningproteindifferent?
Howcanalphahelicalstructurebeusedtotightlycoupletwoproteins?
Wherearethesidechainsinhelicesandinsheets?Mainchainbonds?
Whatareunstructureddomains,andwhydosomeproteinshavethem?
Howdoproteinsfold(aswellasweknowtheprocess)?
Whatmakesthemunfold?Whatexperimentaltreatmentisusuallyused,andwhythatone?
Whatkind(s)ofsecondarystructureisfoundinfilamentousproteins?Wherearefilamentousproteinsfound?
Whatkindsofpropertiescantheyhave?
Whatismeantbyalternativestructuresforproteins?
Whatareproteinfamilies,andwhydotheyexist?
Whatisaligand?Howisitdifferentfromasubstrate?Howdoesitinteractwithaprotein?
Whatisanallostericenzymeinhibitor,andhowisitdifferentfromasubstrateorproduct?
Whatisadisulfidebond,wheredoesitform,andwhatisitspurpose?
Whatisthepurposeofanantibody?HowisanIgGorganized(componentsandinteractions)?
Howdoesanantibodybindamolecule?Whatisthebondmoleculecalled,specificallyforanantibody?
WhichpartsoftheIgGdetermineitsspecificity?
Whatdoenzymesdotoareactionenergetics(e.g.,inanenergydiagram)?
Whataregeneralmechanismsofenzymeaction?
Whatisthedifferencebetweenanenzymesbindingsiteanditsactivesite?Howaretheyrelated?
Whatisacofactorforanenzymeorotherprotein?
Howdoesfeedbackinhibitionworkinametabolicpathway?Whatkindofinhibitionisit?(allosteric)
Whatisaproteinkinase?Whatisitseffectonthestructureandfunctionaprotein?Howisitsactionreversed?
Aretherekinasesthatarenotproteinkinases?Whatdotheydo?
Whatkindsofcovalentmodificationscanoccurtoproteins?
WhatareGproteins?Whatdotheydo?Howaretheyactivatedanddeactivated?
Ifmotorproteinscanmovewithoutinputofenergy,whatisATPhydrolysisusedfor?
Howischromatographydifferentfromelectrophoresis?
Whatarethemajortypesofcolumnchromatography,andwhatphysicalprincipleorinteractioniseachbased
on?
WhatisSDSgelelectrophoresisusedfor?WhatarethechemicalsthatareaddedtoprotiensforSDSgels,and
whataretheirpurposes?
WhatisthenatureoftheinformationgainedbyXraycrystallographyandstructuralNMR?Whatarethe
limitationsofeachmethod?

 

Solution ID:351020 | This paper was updated on 26-Nov-2015

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