Data for membrane mobility of three different proteins...

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Question 4: [8 pts]
Data for membrane mobility of three different proteins (X, Y, and Z) using fluorescent recovery after
photobleaching (FRAP) are shown on the left in the figure below.
Then, the movement of each protein in the membrane is followed through single-particle tracking (SPT). The data
collected is shown on the right side of the image above.
a. [5pts] Assign an SPT profile (A, B, or C) to each of these proteins on the basis of the respective FRAP profiles.
Explain your choice.
b. [3pts] It is important to remember that in each of these experiments, the results reflect a real, physical
difference in the way in which these proteins are situated in/around the plasma membrane. Where do you think
each of these proteins is located? [No need to provide an explanation]
Question 5: [12 pts]
Extremophiles are microorganisms, usually from the Archeal domain that grow under a wide variety of extreme
conditions (temperature, pressure, etc). Some of these extremophiles can grow in temperatures up to 120C, the
sterilization temperature of an autoclave. Identifying the adaptations that allow these microorganisms to thrive at
such high temperatures is an area of active research both for improving industrial processes (i.e. biofuels) and
exobiology. Recent studies of the membrane composition have found that the major lipids in these organisms are
tetraether phospholipids and diether phospholipids. In the diagrams below, the R represents a variety of head
groups.
a. [3pts] Diagram what a membrane made up of diether phospholipids would look
like. Compare this to the eukaryotic cell membrane shown on the right.
Comparison should include: fluidity, transition temperature and structure.
b. [3pts] Diagram what a membrane made up of tetraether phospholipids would look like. Compare this to the
eukaryotic cell membrane shown in question a. Comparison should include: fluidity, transition temperature and
structure.
c. [3pts] Diagram what a membrane made up of tetraether and diether phospholipids would look like. Compare
this to the eukaryotic cell membrane shown in question a. Comparison should include: fluidity, transition
temperature and structure.
d. [3pts] Would you expect either of the phospholipids in the thermophiles membrane to become more or less
saturated as the temperature increases? Explain your answer.

 

Solution ID:351024 | This paper was updated on 26-Nov-2015

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