Recall that it is possible to design a simple in vitro experiment...


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Level of Detail: Only answer needed
Other Requirements: Recall that it is possible to design a simple in vitro experiment to directly test the function of a splicing regulator. Now imagine that you are talking to a fellow researcher and she tells you that she is studying a new splicing regulator she recently discovered. She tells you that she designed
a simple in vitro test where she placed a simple RNA construct with the correct binding sequence, purified spliceosome as well as a little bit of her new splicing regulator. She also tells you that her experiment failed to work as she did not manage to measure any alternative splicing. Assuming that she
did not make any technical mistakes, could you give her some advice on what might going on? Why could this experiment be failing? Is there something that your friend could try to get the experiment to work?


Solution ID:351054 | This paper was updated on 26-Nov-2015

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