RBFox-1 gene in heart disease...

Description

New solution updates


Question

I need homework help with my biomedical research class. Each question should be answered in half a page single spaced. The questions are about research done on the RBFox-1 gene in heart disease. For question number 2, the disease model used was the TAC model in mice. Please answer each question in a half a page each.
Attachment Preview
problem set #2-1.pdf Download Attachment
BR5HASummer2014ProblemSet2
Due:Tuesday,August19,2014
As promised, here is problem set #2. Note that the questions require logical thought and
imagination more than good memory and good note taking (although your notes will certainly
helpyougetideastoanswerthesequestions).Remember,therearenosinglerightanswers.Each
questionisworth12.5points,foratotalof50pointsonthisproblemset.
Pleasedonthesitatetocomebyofficehours,ormakeanappointmentwithmeifyoufeelthata
particularquestionisunclear.
Pleasereadtheseinstructionscarefully
Each question should be answered in no more than half a single spaced page. Therefore the
assignmentshouldnotexceedtwosinglespacedpages.
Dontusefontsmallerthan10pointsothatIcanreadwithoutstrainingmyeyes.
1) In class we discussed the importance of finding a biomarker that can reliably detect heart failure
especially beforemajorsymptomsbecomeobvioustothepatient. To answerthisquestion,imagine
thatanewbiomarkerforheartdiseaseisdiscovered.Youreadthatthisbiomarkerisaproteinwhich
isnormallynotfoundinblood,butwhichappearsin80%ofasymptomaticpeoplewhowilleventually
goontodevelopheartdisease.Thearticleyoureadisverypositiveaboutthisnewbiomarkerandits
potential to save lives, but being a critical thinker you decide to play the role of devils advocate. Is
thereanypotentialdisadvantageofusingthisbiomarker?Wouldyouultimately(afterconsideringthe
prosandcons)beinfavororagainstitsuse?Why?
2)HowdidDr.Wangmodelchronicheartdiseaseinmice?Howdoesthismodelcomparetoactualheart
disease in humans? What are some of the disadvantages of using this disease model? Why was this
diseasemodeluseddespiteitsflaws?
3) One of the surprising findings in this seminar is that the splicing patterns of diseased heart tissue
mirrorthesplicingpatternsofthedevelopingheart(seeslide15).Rememberthatwestilldontknow
exactly what this means, but this should not stop us from thinking about it. For example, we could
makeanargumentthatthesplicingpatternsweseeinthediseasedheartaredamaging(i.e.casing
theactualdamagetotheheart).Alternativelywecouldjustaseasilysaythatthesesplicingpatterns
are protective (i.e. helping minimize damage to the heart). Notice that these two statements are
startingtolooklikealternativehypotheses.So,whatwouldyouchooseasyourinitialhypothesis?Do
you think that the splicing patterns are damaging or protective? How would you justify your
choice? Last, conceptually how would you test your hypothesis? (Notice that I say conceptually, so
pleasedontfocusonexperimentaltechniques.Focusonthelogicofyourexperiment.)
4) Takealookatthehypotheticalresultsseeninfigure1.HereyouseearepresentationofaqRTPCR
experimenttellingyouthattube#2(diseasedheart)containsmoreoftheheartstructuralgenethan
tube#1(healthyheart).Youknowthisbecausethesignalreachesthethresholdfirstintube#2.Ifyou
take a look at figure 2 you notice that this makes perfect sense as there is more heart structural
mRNA in tube #2 to begin with, so you might even feel safe to conclude that indeed the diseased
1
heart produces more of the heart structural mRNA and that this change in expression might be
associated with heart disease. Why would this conclusion be flawed? How can the housekeeping
genehelpyouprovetheflaw?
[Note:ahousekeepinggeneisoneofthemanygenesthatareexpressedmoreorlessequallyinallcells
andhelpsmaintainthem].

 

Solution ID:351060 | This paper was updated on 26-Nov-2015

Price : $40
SiteLock