Enzymes Temperature pH and Specificity....

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44-0054-00-01 RPT Enzymes Temperature pH and Specificity.docx Download Attachment
Enzymes
Specificity
Temperature
pH
and
Hands-On Labs, Inc. Version 42-0054-00-01
b e r sitn
L R s s a t
a
po A
t
This document is not meant to be a substitute for a formal laboratory report. The Lab
Report Assistant is simply a summary of the experiments questions, diagrams if needed
and data tables that should be addressed in a formal lab report. The intent is to facilitate
students writing of lab reports by providing this information in an editable file which can be
sent to an instructor.
Exercise 1: Enzyme Specificity
sr a ns
Ob tio
e v
Data Table 1: Glucose Concentration
Wells
a
b
c
d
e
Concentration of Glucose
Qu sti ns
e o
A. What determines a persons ability to digest lactose?
B. Which of the wells showed a positive result for glucose? Explain the results.
C. Explain why testing for glucose is used to determine the activity of the enzyme
lactase.
D. Explain the experimental conditions for the five different wells.
able 2: Presence of glucose in wells indicating lactase activity at
various temperatures
Well
Time (min)
Concentration of Glucose
5
a
10
15
20
5
b
10
15
20
5
c
10
15
20
Exercise 2: Enzymes and Temperature
sr a ns
Ob tio
e v
Qu sti ns
e o
A. Graph the effect of temperature on the activity of the enzyme lactase.
B. What happens when an enzyme is boiled? Is this effect reversible?
C. Based on your experiment results, what is the optimal temperature for lactase
function?
D. Explain what happens as far as the effectiveness of the enzyme at the freezing
temperature. Can this effect be overcome when the temperature rises?
Exercise 3: Enzymes and pH
sr a ns
Ob tio
e v
Data Table 3: Glucose in wells a-d indicates enzyme activity at various pH
levels
Well
a
b
c
d
pH
3.5
5.0
6.8
11.0
Concentration of Glucose
Qu sti ns
e o
A. Graph the data placing glucose concentrations on the y-axis and the pH values on
the x-axis.
B. What was the effect of pH on the enzyme lactase? Is this true for all enzymes?

 

Solution ID:351122 | This paper was updated on 26-Nov-2015

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