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Microarray worksheet (8 questions multiple choice)
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Lesson6Laboratory:Microarray
1.Genomicsisthestudyof
a.Thestructureandfunctionofmutationsandhowtheyaltergenetictraits.
b.GenesandtheDNAsequencesbetweengenesandhowtheydetermine
development.
c.TheinformationprovidedbycomputerprogramswhichanalyzesmRNA.
d.Thehumangenomeascomparedtoothervertebrategenomes.
2.Microarraysareaveryusefultoolingenomicsbecausethey
a.HelpscientistsexamineintergeneticDNAbyseparatingitfromgenes.
b.Provideauniquepromoterregionforpolymerasechainreactions.
c.Allowscientiststoexaminethousandsofgenesallatonce.
d.DecreasethetimeittakesforscientiststomakecopiesofDNA.
3.Generally,everycellinourbodycontainsthesame20,000(orso)genes.However,cellsin
ourbodyaredifferentfromeachotherbecausethey
a.Havedifferentgenesturnedonorofftosupportdifferentfunctions.
b.Containdifferentcopiesofgenesfordifferentfunctions.
c.Providedifferentnucleotidebasesforeachdevelopmentalfunction.
d.Functiondifferentlybasedonvaryingproteomics.
TYPETHECORRECT
ANSWERSHERE
(a,b,c,d)
D
a
4.Howcanscientistsdeterminethefunctionofordifferencesbetweencelltypes?Theycan
examinethe
a.Numberofnucleotidebasesingenesversusintergeneticsequences.
b.AmountofmRNAexpressedforeachgeneinacelltype,andthencomparethatinformation
betweencelltypes.
c.Amountofmutationsbetweengenesintheintergeneticspaces.
d.NumberoftRNAcopiesforaparticularcelltype.
b
5.Howisamicroarrayconstructed?Ineachspot,thereare
a.Copiesofallthegenesforanorganism.
b.Multiplecopiesofonegene,eachspothascopiesforadifferentgene.
c.Multiplecopiesofintergeneticsequences,whichbindtogenesinthesamples.
d.Copiesofintergeneticsequences,whichpromotethereplicationofDNAinasample.
C
6.TheexperimentthatbeginsinChapter3ofthesimulationseekstoanswerthequestion
a.Whatisthedifferencebetweenintergeneticspacesincancercellsversushealthycells?
b.WhydodifferentcelltypesexpressdifferentamountsofmRNA?
c.Howdodifferentcancercellsproducedifferentmutations?
d.Whatisthedifferencebetweenhealthycellsandcancercells?
C
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A
7.Whycantdoctorsusecellappearancetodiagnosecancer?
a.Notallcancercellslookdifferentfromhealthycells.
b.Cancercellsaretoosmalltoexamineusingcellappearance.
c.Notallcancercellsareabletobebiopsiedfromthebody.
d.Cancercellschangeappearancewhentakenoutofthebody.
8.Intheexperiment,asolventisaddedtoeachcelltype(healthycellsandcancercells).After
thesampletubecontainingeachcelltypeismixedonthevortex,theRNAisseparatedfromthe
restofthesampleinacentrifuge.WhydoesDNAsettletothebottomofthetubeandRNA
doesnt?
a.RNAismuchlongerthanDNA.
b.RNAisattachedtoproteinsthathelpitstayinsolution.
c.DNAisattachedtobiomoleculesthatweighitdownandhelpitsettletothebottom.
d.DNAismuchlongerthanRNA.
9.WhatfeaturedoesmRNAhavethattRNAandrRNAdonot?mRNAalways
a.ContainsaGABAbox.
b.ContainsaTATAsequence.
c.EndswithaGtail.
d.EndswithapolyAtail.
10.HowdothebeadsinthecolumnseparatemRNAfromallotherRNA?Thebeadscontain
a.SequencesthatmagneticallyseparatethemRNA.
b.Agluelikesubstancederivedfromspiderwebs.
c.PolyTs.
d.AsequenceofuracilsthatbindtothePolyAtail.
11.AfteryouisolatemRNA,youhavetomakeaDNAcopy.WhycantwejustusemRNA?
a.DNAismuchmorestablethanmRNA.
b.Wehavetoaddafluorescentlabelthatwillallowustoseethesample.
c.mRNAwilleventuallytransformintotRNAmakingitunusable.
d.AandB
12.Scientistscallhybridizationthekeytomicroarrays.Hybridizationoccurswhen
a.TwocomplimentarystrandsofDNAfromdifferentsourcesbindtoeachother.
b.PolyAtailsbindtoPolyTs.
c.DifferentspeciesinterbreedandcreatenewDNAbasepairings.
d.TwostrandsofidenticalDNAbindwithoutusingthetraditionalnucleotidepairs.
13.Whenyouscanthemicroarrayinthescanner,thedatashowsomedarkspots.Whatdo
theserepresent?
a.TheDNAthathasbeenreplicatedinhealthycells.
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b.ThemRNAthatwaswashedawayinthewashingsolution.
c.TheDNAthatwasnottranscribedandexpressedinhealthycells.
d.ThemRNAthatwasnotboundbyOligodtailsinthebeads.
14.Whenyouscanthemicroarrayinthescanner,somespotsareyellowandrepresentplaces
wherethegenewasexpressedinbothhealthyandcancercells.Thesespotstellus
a.Wheretolookformutations.
b.WhereDNAhybridizedincancercells.
c.ThatDNAexpressiondidntchangeinthesegeneswhencanceroccurred.
d.Thatthemicroarraydidntworkinthesegenes.
15.Inourexample,gene6219mRNAismadeinbothhealthyandcancerouscells,however
proteinsareonlytranslatedfromthatmRNAinhealthycells.Microarrayanalysis
a.Showsusthisdefectbymakingyellowspots.
b.Cannotshowusthisdefect,whichisalimitationofthistypeofanalysis.
c.Showusthisdefectbymakingredspots.
d.Cannotshowusthisdefect,whichisabenefitofthistypeofanalysis.

 

Solution ID:351211 | This paper was updated on 26-Nov-2015

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